C0871

Immunoprecipitation
Rabbit Anti-Cullin 3 (Cat. No. C0871) was used to immunoprecipitate Cullin 3 from rat brain whole extract. Detection antibody: Rabbit Anti-Cullin 3 (C-terminal) antibody (Cat. No. C9745).
Lanes
1. 5 μg antibody
2. Negative control: 5 μg non-relevant antibody
3. Negative control: without precipitating antibody

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C0871

Sigma

 

Anti-Cullin 3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym:Anti-CUL3

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Description

Immunogensynthetic peptide corresponding to amino acids 194-211 of human Cullin 3, conjugated to KLH. The corresponding sequence is identical in mouse and rat.
Physical formSolution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Properties

antibody formaffinity isolated antibody
clone(polyclonal)
formbuffered aqueous solution
mol wtantigen mol wt ~80 kDa
species reactivitymouse, human, rat
concentration~1.0 mg/mL
application(s)immunoblotting: 1-2 μg/mL using whole extract of HEK-293T cells expressing human Cullin 3
 immunoprecipitation: 5-10 μg using lysate of rat brain
 indirect immunofluorescence: 5-10 μg/mL using mouse 3T3 cells
Gene Informationhuman ... CUL3(8452)
 rat ... Cul3(301555)
 mouse ... Cul3(26554)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany1

Related Products

Related productC9745, Anti-Cullin 3 (C-terminal) antibody produced in rabbit